小金蝠蛾幼蟲β-1，3-葡聚糖識別蛋白βGRP1基因的分子特征與生物信息學(xué)分析

賀元川 羅昌樹 游華建 陳仕江

摘要 [目的]探索冬蟲夏草寄主昆蟲小金蝠蛾幼蟲的β-1，3-葡聚糖識別蛋白βGRP1基因的結(jié)構(gòu)特征與進(jìn)化關(guān)系。[方法]通過PCR法克隆獲得小金蝠蛾幼蟲βGRP1基因的編碼序列，并采用Protparam、NetPhos 2.0 Server cDNA、MEGA等軟件分析βGRP1蛋白的理化特征和進(jìn)化分析。[結(jié)果]小金蝠蛾幼蟲βGRP1基因編碼序列1 593 bp，推斷可編碼530氨基酸組成的蛋白，該蛋白為分泌型蛋白，含有20個氨基酸的信號肽，包含2個功能結(jié)構(gòu)域，該結(jié)構(gòu)域與葡聚糖的識別密切相關(guān);同源分析和分子系統(tǒng)進(jìn)化樹分析表明，鱗翅目昆蟲中的βGRPs基因家族分為4個進(jìn)化分枝，其中小金蝙蝠蛾與勾蝠蛾為單獨的一個進(jìn)化分枝，小金蝠蛾幼蟲βGRP1進(jìn)化較鉤蝠蛾早，介于I型和Ⅲ型分支。[結(jié)論]該蛋白具有識別β-1，3-葡聚糖的結(jié)構(gòu)域，并可能通過絲氨酸磷酸化激活酚酶源級聯(lián)系統(tǒng)，啟動免疫，為進(jìn)一步探索βGRP1蛋白的免疫功能奠定基礎(chǔ)。

關(guān)鍵詞 冬蟲夏草;小金蝠蛾;β-1，3-葡聚糖識別蛋白;先天免疫

中圖分類號 Q 966? 文獻(xiàn)標(biāo)識碼 A? 文章編號 0517-6611（2021）19-0095-04

doi：10.3969/j.issn.0517-6611.2021.19.023

開放科學(xué)（資源服務(wù)）標(biāo)識碼（OSID）：

Molecular Characteristics and Bioinformatics Analysis of β-1，3-glucan Recognition Protein βGRP1 Gene of ?Hepialus xiaojinensis ?Larvae

HE Yuan-chuan1，2， LUO Chang-shu1， YOU Hua-jian1，2 et al

（1. Chongqing Academy of Chinese Materia Medica， Chongqing 400065; 2. Gongga Chinese Caterpillar Fungus Industry Co.， Ltd.， Kangding， Sichuan 626000）

Abstract [Objective]The study aimed to explore the structural characteristics and evolutionary relationship of the β-1， 3-glucan recognition protein βGRP1 gene of the larvae of the host insect ?Hepialus xiaojinensis . [Method]The coding sequence of the βGRP1 gene of ?Hepialus ?larvae was cloned by PCR， and the physical and chemical characteristics and evolutionary analysis of βGRP1 protein were analyzed by Protparam， NetPhos 2.0 Server cDNA， MEHA and other methods. [Result]The coding sequence of βGRP1 of the larvae of ?Hepialus xiaojinensis ?is 1 593 bp， which is inferred to encode a protein composed of 530 amino acids. This protein is a secreted protein， contains a signal peptide of 20 amino acids and contains 2 functional domains， which probably combined with dextran. The analysis results of molecular phylogenetic tree showed that the ?Lepidopteran ?insects βGRPs gene family was divided into 4 evolutionary branches. The evolution of βGRP1 of the larvae of ?Hepialus xiaojinensis ?is earlier than that of Hepialus pu， which lies between type I and Ⅲ. [Conclusion] The study indicates that the protein has a domain that recognizes β-1， 3-glucan， and may activate the phenolase-derived cascade system through serine phosphorylation to initiate immunity， which supply protein foundation for research the immune function of βGRP1.

Key words ?Ophiocordyceps sinensis ; Hepialus xiaojinensis ;β-1， 3-glucan recognition protein;Innate immunity