


[摘要] 目的 了解血液培養(yǎng)的常見病原菌種類和藥敏情況,為臨床正確選用抗生素提供依據(jù)。 方法 對鄭州大學第三附屬醫(yī)院2010年1月~2011年12月的454份血培養(yǎng)陽性標本,采用 BACT/ALERT 120全自動血培養(yǎng)儀培養(yǎng),VITEK32全自動細菌鑒定儀進行鑒定,MIC法進行細菌藥敏試驗。 結(jié)果 454例血培養(yǎng)陽性標本共分離出細菌462株,其中革蘭陽性球菌268株,革蘭陰性桿菌139株,革蘭陽性桿菌16株,真菌10株;居前4位的感染病原菌依次是表皮葡萄球菌、金黃色葡萄球菌、大腸埃希氏菌、白色念珠菌。藥敏結(jié)果顯示:未發(fā)現(xiàn)耐萬古霉素的革蘭陽性球菌,革蘭陰性桿菌對碳青霉烯類抗生素高度敏感。 結(jié)論 及時了解血培養(yǎng)結(jié)果可以對臨床抗菌治療提供依據(jù),提高治愈率,降低死亡率有重要的臨床意義。
[關(guān)鍵詞] 血培養(yǎng);病原菌;藥敏試驗
[中圖分類號] R446.1 [文獻標識碼] B [文章編號] 2095-0616(2012)21-116-02
Analysis of 462 cases of positive blood culture results
LIU Litao
Department of Urology Surgery,Xiayi County People's Hospital,Xiayi 476400,China
[Abstract] Objective To investigate the distribution and drug resistance of pathogenic bacteria isolated from blood cultures,in order to provide evidence for rational use of antibiotics in clinic. Methods From January 2010 to December 2011,a total of 454 positive blood culture samples in the third affiliated hospital of Zhengzhou university were cultured by BACTEC9120 system,the isolates were identified with VITEK32 automated bacteria identification instrument,drug sensitive tests were performed by MIC method. Results 462 microorganisms were isolated from 454 positive blood culture samples, which contain 268 strains of gram-positive cocci,139 strains of gram-negative bacilli,16 strains of gram-positive bacilli,and 10 strains of fungi;The main pathogenic bacteria were staphylococcus epidermidi,staphylococcus aureus,escherichia coli and vandida albicans.The data showed that it was highly sensitive of vancomycin to gram-positive cocci and carbapenem to gram-negative bacilli. Conclusion It is important to comprehend the blood culture results promptly for the selection of antimicrobial agents in order to increase cure rate and decrease mortality.
[Key words] Blood culture;Pathogens;Susceptibility test
血培養(yǎng)是診斷敗血癥的重要手段,對明確病原菌、指導臨床合理應用抗生素非常重要。現(xiàn)對鄭州大學第三附屬醫(yī)院2010年1月~2011年12月血培養(yǎng)中的陽性結(jié)果的細菌分布和對常用抗生素的敏感性進行分析,期望為臨床提供借鑒。
1 資料與方法
1.1 一般資料
選取鄭州大學第三附屬醫(yī)院2010年1月~2011年12月疑為血行感染的住院患者血培養(yǎng)陽性結(jié)果462例進行病原學和藥敏情況分析。其中男297例(64.3%),女165例(35.7%);新生兒科202例(43.7%),小兒呼吸內(nèi)科119例(25.7%),小兒神經(jīng)內(nèi)科34例(7.4%),小兒血液內(nèi)科27例(5.8%),小兒骨科23例(5.0%),小兒心外科57例(12.3%)。
1.2 標本采集
在患者寒戰(zhàn)或高熱時,無菌操作抽取靜脈血3~5 mL迅速注入到血培養(yǎng)瓶中,立即送檢。……