原發性肝癌患者用血清、血漿標本測定循環VEGF濃度的差別

[摘要] 目的 探索分析循環中VEGF濃度在血清和血漿中的差別，及其與血小板計數的關系。 方法 從20例未曾接受過治療的原發性肝癌患者分別采取血清和血漿標本使用酶聯免疫吸附法測定VEGF濃度做自身對照比較。同時分析血小板計數與血清VEGF和血漿VEGF的相關性。 結果 血清VEGF濃度顯著高于血漿VEGF（P=0.000，Z=-3.920），血清和血漿VEGF濃度顯著正相關（R=0.883，P=0.000）。血清、血漿VEGF濃度均與血小板計數正相關（R=0.652，P=0.002；R=0.487，P=0.029），血漿VEGF濃度與血小板計數相關性較弱。 結論 由于受外周血血小板計數的影響較小，血漿VEGF可能更適于監測原發性肝癌患者的血管生成狀態。

[關鍵詞] 癌；肝細胞；血管內皮生長因子；血清；血漿；酶聯免疫吸附測定

[中圖分類號] R735.7 [文獻標識碼] B [文章編號] 2095-0616（2012）21-108-02

Difference between using serum and plasma samples in measuring circulating VEGF concentration of patients with primary liver cancer

CHENG Hongtao GUO Chenyang LI Hailiang XIAO Jincheng HU Hongtao ZHENG Lin

ZONG Dengwei YU Pu

Department of Radiology， Affiliated Cancer Hospital of Zhengzhou University，Zhengzhou 450008，China

[Abstract] Objective To explore and analyze the difference between serum and plasma in the circulating VEGF concentration and its relationship with platelet count. Methods Serum and plasma samples were collected from 20 untreated patients with primary liver cancer and the enzyme-linked immunosorbent assay was used to measure the VEGF concentration for self control comparison. Meanwhile the correlation between the platelet count and the serum VEGF and plasma VEGF was analyzed. Results The serum VEGF concentration was significantly higher than the plasma VEGF concentration（P=0.000， Z=-3.920）， with positive correlation between them （R=0.883，P=0.000）. Both serum and plasma VEGF concentrations were positively correlated to the platelet count（R=0.652，P=0.002；R=0.487，P=0.029）， but the correlation between the serum VEGF concentration and the platelet count was weak. Conclusion Due to the small influence from peripheral blood platelet count， plasma VEGF may be more suitable for the monitoring of angiogenesis state of patients with primary liver cancer.

[Key words] Cancer； Liver cells； Vascular endothelial growth factor； Serum； Plasma； Enzyme-linked immunosorbent assay

血管內皮生長因子（vascular endothelial growth factor，VEGF）在肝細胞肝癌的新血管形成過程中具有重要作用，對肝癌患者的預后有重要影響。多數血管生成因子是由腫瘤分泌的可擴散的可溶性肽鏈，理論上循環中的血管生成因子水平能夠反映腫瘤血管生成的總體活性，通過外周血測定VEGF也可以反映出腫瘤血管生成活性[1]。用血清抑或血漿標本測定外周血VEGF可能獲得不同的結果。本研究采用酶聯免疫吸附法對兩種標本測定外周血VEGF進行自身對照研究，初步探索更適于測定外周血VEGF的標本類型。

1 資料與方法

1.1 一般資料

2010年11月～2012年6月同時測定血清和血漿VEGF濃度的原發性肝癌患者20例，其中男14例，女6例，平均年齡為（59.4±10.9）歲。所有患者之前未接受過任何治療，均于清晨空腹時于肘靜脈抽取靜脈血。……