宮頸癌EGFR和VEGF的表達(dá)及相關(guān)性研究

周素英+陳國榮+馮國飛+潘丹+張品南+楊小敏+夏作利

[摘要] 目的 探討宮頸癌中EGFR及VEGF的表達(dá)與宮頸癌的關(guān)系及其相關(guān)性。方法 采用免疫組化EnVision法檢測80例宮頸癌組織芯片中EGFR和VEGF的表達(dá)，分析其與宮頸癌臨床病理特征的關(guān)系及其間相關(guān)性。結(jié)果 宮頸癌中的EGFR和VEGF陽性表達(dá)率分別為94.8%、79.1%，癌旁組織中的表達(dá)率分別為15%、20%。兩者在兩種組織中的表達(dá)有顯著差異（P<0.01）。EGFR表達(dá)與腫瘤大小、組織類型、病理分級(jí)、浸潤深度、淋巴結(jié)轉(zhuǎn)移、臨床分期有關(guān)（P<0.05）；VEGF與腫瘤大小、淋巴結(jié)轉(zhuǎn)移、臨床分期有關(guān)（P<0.05）；EGFR和VEGF表達(dá)兩者之間有顯著性相關(guān)性（r=0.2548，P<0.05）。結(jié)論 EGFR和VEGF高表達(dá)與宮頸癌的生長、浸潤轉(zhuǎn)移及預(yù)后有相關(guān)性，兩者在宮頸癌的發(fā)展中可能存在協(xié)同作用。

[關(guān)鍵詞] 宮頸癌；EGFR；VEGF；免疫組織化學(xué)；組織芯片；靶向治療

[中圖分類號(hào)] R737.33 [文獻(xiàn)標(biāo)識(shí)碼] B [文章編號(hào)] 1673-9701（2014）02-0068-04

Expression and correlation of EGFR and VEGF in cervical carcinoma

ZHOU Suying1 CHEN Guorong2 FENG Guofei1 PAN Dan1 ZHANG Pinnan1 YANG Xiaomin1 XIA Zuoli1

1.Department of Pathology， the Peoples Hospital of Wenzhou City in Zhejiang Province， Wenzhou 325200， China；

2.Department of Pathology， the First Affiliated Hospital of Wenzhou Medical College， Wenzhou 325000， China

[Abstract] Objective To study the relationship between the expressions of EGFR， VEGF in cervical carcinoma and to observe the correlation between EGFR and VEGF in them. Methods The expressions of EGFR and VEGF were studied by immunohistochemical EnVision in tissue microarray containing 80 cervical carcinoma tissues and the corresponding adjacent tissues. The relationship between EGFR and VEGF expression and the chinicopathological features was analyzed. Results The positive rates of EGFR and VEGF were 94.8% and 79.1% respectively in cervical carcinoma. However they were 15% and 20% respectively in the corresponding adjacent tissues. Expressions of EGFR and VEGF proteins were significantly higher in cervical carcinoma than those in adjacent tissues （P<0.01）. The overexpression of EGFR was found significantly associated with the tumer size，tissue type，differentiation degree， knubbly invasive depth，lymphnode metastasis and clinical stage（P<0.05）. That of VEGF found significantly associated with the tumer size，lymphnode metastasis and clinical stage （P<0.05）. Besides， the overexpression of EGFR was correlated with the overexpression of VEGF （r=0.2548， P<0.05）. Conclusion The overexpression of EGFR and VEGF in cervical carcinoma are closely correlated with the growth， invasion and metastasis， prognosis， and maybe have synergistic effect in development of cervical carcinoma.

[Key words] Cervical carcinoma； Epidermal growth factor receptor（EGFR）； Vascular endothelial growth factor（VEGF）； Immunohistochemistry； Tissue microarray； Targeted therapy

宮頸癌是發(fā)生在全球女性中第二大常見的惡性腫瘤[1]，其發(fā)病率有逐年上升且發(fā)病年輕化的趨勢。表皮生長因子受體 （epidermal growth factor receptor，EGFR）是宮頸癌發(fā)生發(fā)展的重要因素，它與相應(yīng)的配體結(jié)合后，形成同源二聚體或異源二聚體，使受體的酪氨酸激酶，啟動(dòng)一系列調(diào)節(jié)細(xì)胞各方面功能的下游信號(hào)轉(zhuǎn)導(dǎo)通路，包括細(xì)胞增殖、凋亡、血管發(fā)生、吸附和運(yùn)動(dòng)性，在癌組織中，這些通路調(diào)節(jié)障礙，可致使細(xì)胞過度增殖、轉(zhuǎn)移和耐藥[2]。endprint

血管內(nèi)皮生長因子（vascular endothelial growth factor，VEGF）是目前發(fā)現(xiàn)的最重要的促血管生長因子，有促進(jìn)血管內(nèi)皮細(xì)胞分化、增生、遷移和浸潤的作用[3]，在多種腫瘤中VEGF的表達(dá)增高，并且其水平的高低與腫瘤的某些生物學(xué)行為有密切關(guān)系。本文對(duì)構(gòu)建含有80例宮頸癌和癌旁組織標(biāo)本的組織芯片進(jìn)行免疫組化EnVision法檢測EGFR、VEGF的表達(dá)，以探討宮頸癌發(fā)生發(fā)展中EGFR、VEGF表達(dá)與宮頸癌臨床病理特征的關(guān)系和相關(guān)性，尋找宮頸癌抗腫瘤治療的靶點(diǎn)。

1資料與方法

1.1臨床資料

隨機(jī)選擇溫州市人民醫(yī)院病理科2003～2009年間80例宮頸癌根治術(shù)石蠟包埋組織蠟塊。……