mdig基因在人胰腺癌中的表達(dá)及其促癌作用

羅和生+占婷+田霞+黃曉東

[摘要] 目的 研究mdig基因在胰腺癌細(xì)胞中的表達(dá)情況，探討mdig與胰腺癌發(fā)生發(fā)展的關(guān)系及其作用的可能機(jī)制。 方法 采用實(shí)時(shí)熒光定量聚合酶鏈反應(yīng)（Q-PCR）和Western blot方法檢測(cè)mdig在胰腺癌細(xì)胞PANC1和MIAPaca2與正常胰腺導(dǎo)管上皮細(xì)胞HPDE6c7中表達(dá)的差異。將轉(zhuǎn)染mdigsi RNA質(zhì)粒的胰腺癌細(xì)胞PANC1和MIAPaca2設(shè)為實(shí)驗(yàn)組，將轉(zhuǎn)染空載質(zhì)粒的胰腺癌細(xì)胞PANC1和MIAPaca2設(shè)為對(duì)照組，用CCK8法和流式測(cè)凋亡法分別檢測(cè)兩組細(xì)胞的增殖和凋亡情況；用Western blot檢測(cè)兩組細(xì)胞的Sox2基因表達(dá)情況。 結(jié)果 與正常胰腺導(dǎo)管上皮細(xì)胞HPDE6c7相比，mdig基因在胰腺癌細(xì)胞PANC1和MIAPaca2中表達(dá)均上調(diào)（P < 0.05）。與對(duì)照組相比，實(shí)驗(yàn)組細(xì)胞的增殖率下降（P < 0.05），凋亡率增高（P < 0.05），Sox2的表達(dá)下調(diào)（P < 0.05）。 結(jié)論 mdig在胰腺癌細(xì)胞中表達(dá)升高，并可能通過介導(dǎo)原癌基因Sox2發(fā)揮促癌作用。

[關(guān)鍵詞] 胰腺癌；礦物粉塵誘導(dǎo)基因；增殖；凋亡

[中圖分類號(hào)] R735 [文獻(xiàn)標(biāo)識(shí)碼] A [文章編號(hào)] 1673-7210（2017）03（b）-0012-04

Expression of mdig gene in human pancreatic carcinoma and its role in promoting cancer

LUO Hesheng1 ZHAN Ting1 TIAN Xia HUANG Xiaodong2

1.Department of Gastroenterology， People's Hospitial of Wuhan University， Hubei Province， Wuhan 430060， China； 2. Department of Gastroenterology， Tongren Hospital of Wuhan Univerity， Hubei Province， Wuhan 430060， China

[Abstract] Objective To study the expression of mdig in pancreatic cancer cells， in order to explore the relationship between mdig and the development of pancreatic cancer and its possible mechanism. Methods The expression of mdig in pancreatic cancer cells and normal pancreatic ductal epithelial cells were detected by Q-PCR and Western blot. The pancreatic cancer cells PANC1 and MIAPaca2 transfected with mdigsi RNA plasmid were designated as the experimental group， and the pancreatic cancer cells PANC1 and MIAPaca2 transfected with empty plasmid were designated as control group， the proliferation of two groups of cells was detected by CCK8， the apoptosis of two groups of cells was detected by flow cytometry， the expression of Sox2 in two groups of cells was detected Western blot. Results Compared with normal pancreatic ductal epithelial cells HPDE6c7， the expression of mdigin pancreatic cancer cells PANC1 and MIAPaca2 was up-regulatede. Compared with the control group， the proliferation rate of the experimental group was decreased （P < 0.05）， the apoptosis rate was increased （P < 0.05）， and the expression of Sox2 was down regulated （P < 0.05）. Conclusion The expression of mdig in pancreatic cancer cells increases， and mdig may regulate the expression of Sox2 to promoting the occurrence of pancreatic cancer.

[Key words] Pancreatic cancer； Mdig； Proliferation； Apoptosis

近年來，胰腺癌的發(fā)生率和死亡率逐漸升高，已成為癌癥致死的第4位病因[1]，它的侵襲性生長和早期轉(zhuǎn)移擴(kuò)散使其成為最具侵襲性的腫瘤之一，約40%患者在確診時(shí)已經(jīng)發(fā)生局部或遠(yuǎn)處轉(zhuǎn)移，失去根治性手術(shù)的機(jī)會(huì)[2]。隨著研究的深入發(fā)現(xiàn)，腫瘤的發(fā)生往往是多種基因共同作用的結(jié)果[3-4]因此可以通過研究與胰腺癌發(fā)病密切相關(guān)的基因，從而在分子水平上尋找其中的關(guān)鍵基因并開發(fā)相應(yīng)的靶向治療。礦物粉塵誘導(dǎo)基因（mineral dust induced gene，mdig）于2005年作為一種新肺癌基因被發(fā)現(xiàn)[5]，隨后被證實(shí)與mina53基因是同一種基因，而mina53基因是原癌基因c-myc的靶基因[6]。越來越多的研究證實(shí)mdig基因是一種重要的腫瘤相關(guān)基因，與某些腫瘤的發(fā)生與發(fā)展密切相關(guān)，并且已經(jīng)有報(bào)道發(fā)現(xiàn)mdig在人類結(jié)腸癌[7]，食管鱗狀細(xì)胞癌[8]、肝細(xì)胞癌[9]、膽管細(xì)胞癌[10]表達(dá)均升高。然而，目前只有極少數(shù)關(guān)于mdig基因在胰腺癌組織中的表達(dá)情況的報(bào)道，而且其表達(dá)情況的定量研究及其與胰腺癌發(fā)生發(fā)展的關(guān)系還未見報(bào)道。本研究擬探討mdig基因在胰腺癌細(xì)胞中表達(dá)狀況及其促癌作用并探討其發(fā)揮作用的可能機(jī)制。