玉屏風散合四物湯對慢性阻塞性肺疾病大鼠氣道7種細菌的影響

彭靜+羊忠山++李玉卿++袁嘉麗

[摘要] 目的 觀察玉屏風散合四物湯對慢性阻塞性肺病（COPD）大鼠氣道肺炎克雷伯菌、銅綠假單胞菌、流感嗜血桿菌、腸桿菌、鏈球菌、奈瑟菌、乳酸桿菌的影響作用，為玉屏風散合四物湯治療COPD提供一定的實驗依據。 方法 按隨機數字表將50只SPF級雄性Wistar大鼠分為正常組，模型組，玉屏風散合四物湯高、中、低劑量組，每組各10只。通過氣管滴注脂多糖加煙熏的方法建立COPD大鼠模型。造模結束后，給藥組分別給予不同濃度玉屏風散合四物湯灌胃，正常組和模型組以無菌蒸餾水灌胃，每日1次，連續30 d。收集所有大鼠肺組織勻漿進行各種細菌的高通量測序檢測，取左肺組織制作病理切片。 結果 ①與正常組相比，模型組乳酸桿菌含量顯著降低，其余6種細菌含量均顯著升高，差異有統計學意義（P < 0.05）；②與模型組相比，玉屏風散合四物湯高、中、低劑量組的肺炎克雷伯菌、銅綠假單胞菌含量顯著降低，高劑量組的流感嗜血桿菌、腸桿菌、鏈球菌、奈瑟菌含量均顯著降低，差異有統計學意義（P < 0.05），而中、低劑量組差異無統計學意義（P > 0.05）；③與模型組相比，玉屏風散合四物湯低、中劑量組的乳酸桿菌含量顯著升高，差異有統計學意義（P < 0.05），而高劑量組差異無統計學意義（P > 0.05）。 結論 玉屏風散合四物湯可使COPD大鼠肺炎克雷伯菌、銅綠假單胞菌、流感嗜血桿菌、腸桿菌、鏈球菌、奈瑟菌含量明顯減少，乳酸桿菌含量增多，這可能是其治療COPD的重要機制之一。

[關鍵詞] 玉屏風散；四物湯；慢性阻塞性肺疾病；細菌

[中圖分類號] R563 [文獻標識碼] A [文章編號] 1673-7210（2017）11（c）-0013-04

Effects of Yupingfeng San and Siwu Decoction on seven kinds of bacteria in rats with chronic obstructive pulmonary disease

PENG Jing YANG Zhongshan LI Yuqing YUAN Jiali

Basic Medical College， Yunnan University of Traditional Chinese Medicine， Yunnan Province， Kunming 650500， China

[Abstract] Objective To observe the effect of Yupingfeng San and Siwu Decoction on Klebsiella pneumoniae， Pseudomonas aeruginosa， Haemophilus influenzae， Enterobacter， Streptococcus， Neisseria and Lactobacillus in rats with chronic obstructive pulmonary disease （COPD）， in order to provide experimental basis for Yupingfeng San and Siwu Decoction in the treatment of COPD. Methods Fifty SPF male wistar rats were divided into 5 groups， incliding normal group， model group， Yupingfeng San and Siwu Decoction high dose， middle dose and low dose groups， with 10 cases in each group. The COPD rat model was established by intratracheal instillation of lipopolysaccharide plus smoked methods. After molding， the rats in the dose groups were given to different concentrations of Yupingfeng San and Siwu Decoction by gavage， while the rats in the normal group and model group were fed with sterile distilled water， once time a day for 30 days. All the lung tissues of rats were collected， to for high-throughput sequencing of various bacteria， and the left lung tissue was used to make pathological sections. Results ①Compared with the normal group， the content of Lactobacillus in the model group was significantly decreased， and the contents of the other six bacteria were significantly increased， with statistically significant difference （P < 0.05）.②Compared with model group， the contents of Klebsiella pneumoniae， Pseudomonas aeruginosa in the Yupingfeng San and Siwu Decoction high， middle and low dose groups were significantly reduced， the contents of Haemophilus influenzae， Enterobacter， Streptococcus， Neisseria in the high dose group were significantly reduced， with statistically significant difference （P < 0.05）， but there was no significant difference between the middle and low dose groups （P > 0.05）.③The contents of Lactobacillus in the Yupingfeng San and Siwu Decoction low and middle dose groups was significantly increased， compared with model group， with statistically significant difference （P < 0.05）， there was no significant difference between high dose group and model group （P < 0.05）. Conclusion The contents of Klebsiella pneumoniae， Pseudomonas aeruginosa， Haemophilus influenzae， Enterobacter， Streptococcus and Neisseria can be significantly reduced in rats with COPD by Yupingfeng San and Siwu Decoction， the content of Lactobacillus increasing， which may be one of the important mechanisms for the treatment of COPD .endprint

[Key words] Yupingfeng San； Siwu Decoction； Chronic obstructive pulmonary disease； Bacterial

慢性阻塞性肺疾病（chronic obstructive pulmonary disease，COPD）是常見的呼吸系統疾病，嚴重危害患者的身心健康，其氣流受限不完全可逆、呈進行性發展，與肺對吸入煙草煙霧等有害氣體或顆粒的異常炎性反應有關[1]。很多研究表明，細菌定植與COPD的發病及加重密切相關，有效控制細菌感染是治療COPD的重要手段之一[2-4]。……